Patricio Arce

Fono oficina: (56-2) 354-2897
Laboratorio: (56-2) 354-2893
Ubicación oficina: Edificio 210, Piso 7

E- mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Profesor Asociado

Departamento de Genética Molecular y Microbiología,

Facultad de Ciencias Biológicas,

Pontificia Universidad Católica de Chile

Alameda 340, Santiago, Chile

Estudios

Profesor de Ciencias Naturales y Biologia, Pontificia Universidad Católica de Chile. (1982)

Licenciado en Ciencias Biológicas, Pontificia Universidad Católica de Chile (1986)

Doctor en Ciencias mención Ingeniería Genética Vegetal. CINVESTAV-Irapuato, México, (1994)

Postdoctorado, The Scripps Research Institute, La Jolla , C.A., USA. (1995)

Especialización: Virología Molecular Vegetal. Transformación de Plantas. Genómica Vegetal en Vides.

Premios y Distinciones

Premio al patentamiento en Biotecnología vegetal. OTRI-Chile (2001, 2010,2011)
Premio John and Alice Taylor. Becario destacado de la red Latinoamericana de Botánica (2004)

Cursos

Pregrado

BIO225C	Fisiología y Bioquímica Vegetal
BIO364C	Biotecnología Industrial
BIO364I	Desarrollo y evaluación de proyectos en Biotecnología Industrial

Líneas de Investigación

Las plantas son afectadas por numerosos patógenos siendo las enfermedades causadas por virus unas de las más frecuentes y de difícil control. La mayor parte de los virus que infectan plantas son de tipo RNA de hebra sencilla, y se transmiten mediante vectores biológicos o de manera mecánica. Una vez dentro de la célula vegetal los virus se replican en el sitio de infección y se desplazan por movimiento célula a célula a través de los plasmodesmos hasta llegar al sistema vascular, por el que se distribuyen a toda la planta. Estamos interesados en caracterizar el desplazamiento viral y los efectos que estas infecciones causan en las plantas. Para estos estudios utilizamos la planta modelo de Arabidopsis thaliana y la vid, Vitis vinifera, la especie frutal de mayor importancia en Chile. Mediante experimentos de genómica funcional hemos identificado numerosos genes afectados por la infección, y estamos actualmente identificando y caracterizando genes claves en la respuesta de la planta. Nuestros resultados muestran que genes relacionados con el proceso de senescencia se inducen en tejidos verdes de las plantas infectadas. En los frutos de la vid el proceso de maduración también se ve afectado por la infección viral (mayor acidez, menor contenido de sólidos solubles, menor concentración de antocianos) lo que se correlaciona con una disminución en los transcritos de genes de la vía fenilpropanoide y de transportadores de azúcares, entre otros. Una de las aplicaciones de nuestra investigación es la generación de plantas de vides resistentes a enfermedades virales, la que hemos abordado mediante la estrategia de inducción de silenciamiento viral en portainjertos de vid, con muy buenos resultados en condiciones de laboratorio.

Estamos también trabajando en el estudio del estrés abiótico causado por salinidad, Boro y Aluminio en plantas utilizando el modelo de Arabidopsis y también plantas de interés agrícola como los cítricos y el arándano. Hemos identificado genes inducidos por el exceso de B y Al, y estamos trabajando en la generación de portainjertos de cítricos tolerantes a salinidad mediante modificación genética. La sobre-expresión de genes y la complementación en mutantes de la planta modelo Arabidopsis thaliana son herramientas que se utilizan para dilucidar la función de genes de plantas de interés.

1 - Compatible GLRaV-3 viral infection inhibits berry ripening by decreasing sugar accumulation and anthocianin biosynthesis in Vitis vinifera
Autor(es): Vega A, Gutierrez R, Peña-Neira, Cramer G & Arce-Johnson P
Año: 2011. In press
Plant Molecular Bioloy
2 - Genome wide analysis of the set domain group family in grapevine
Autor(es): Aquea F, Vega A, Timmermann T, Poupin MJ & Arce-Johnson P
Año: 2011. 30: 1087-1097
Plant Cell Reproduction
3 - Biochemical and molecular changes in response to aluminum-stress in highbush blueberry (Vaccinum corymbosum L.)
Autor(es): Inostroza-Blancheteau C, Aquea F, Nunes-Nesi A, Alberdi M, Arce-Johnson P, & Reyes-Diaz M
Año: 2011. 12(5): 437-447.
Molecular Plant Pathology
4 - Long term protection against tobacco mosaic virus induced by the marine alga oligosulphated poly-ga in tobacco plants
Autor(es): Vera J, Castro J, Gonzalez A, Barrientos H, Matsuhiro B, Arce P, Zuñiga G & Moenne
Año: 2011. 12 (5) 437-447
Molecular Plant Pathology
5 - Identification of aluminium-regulated genes by c-DNA AFLP analysis in roots of two contrasting genotypes of highbush blueberry (Vaccinum corymbosum L.)
Autor(es): Inostroza-Blancheteau C, Aquea F, Reyes-Diaz M, Alberdi M & Arce-Johnson P
Año: 2011. In press
Molecular Biotechnology
6 - Molecular and physiological strategies to increase aluminum resistance in plants
Autor(es): Dauelsberg P, Matus JT, Poupin MJ, Leiva-Ampuero A, Godoy F & Arce-Johnson P
Año: 2011. In press
Journal of Plant Physiology
7 - Relationship between pollination and fertilization with the expression of genes regulating floral transition, hormone synthesis and early berry development in grapevine (Vitis vinifera L.)
Autor(es): Inostroza-Blancheteau C, Rengel Z, Alberdi M, Mora ML, Aquea F, Arce-Johnson P & Reyes-Diaz M
Año: 2011. In press
Molecular Biology Reports
8 - Analysis of histone acetyltransferase and deacetylase families of Vitis vinifera
Autor(es): Aquea F, Timmermann T & Arce-Johnson P
Año: 2010. 48(2-3): 194-199.
Plant Physiology and Biochemistry
9 - Isolation of WDR and bHLH genes related to flavonoid synthesis in grapevine (Vitis vinifera L.)
Autor(es): Matus JT , Poupin MJ , Canon P, Bordeu E , Alcalde JA & Arce-Johnson P
Año: 2010. 72(6): 607-620.
Plan Molecular Biology
10 - TRAUCO, a Trithorax-group gene homologue, is required for early embryogenesis in Arabidopsis thaliana
Autor(es): Aquea F, Johnston AJ, Canon P, Grossniklaus U & Arce-Johnson P
Año: 2010. 61(4): 1215-1224.
Journal of experimental Botany

11 - Mapping aluminum tolerance loci in cereals: A tool available for crop breeding
Autor(es): Inostroza-Blancheteau C, Soto B, Ibanez C, Ulloa P, Aquea F, Arce-Johnson P & Reyes-Diaz M
Año: 2010. 13(4): 1-12.
Electronic Journal of Biotechnology
12 - Molecular characterization of a Trithorax-group homologue gene from Pinus radiata.
Autor(es): Aquea F, Matte JP, Gutierrez F, Rico S, Lamprecht M & Sanchez C
Año: 2009. 28(10): 1531-1538.
Plant Cell Reproduction
13 - Post-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera
Autor(es): Matus JT, Loyola R, Vega A, Peña-Neira A, Bordeu E, Arce-Johnson P & Alcalde A
Año: 2009. 60(3): 853-867.
Journal of Experimental Botany
14 - Somatic embryogenesis and efficient regeneration of Vitis vinifera L., cv. Carmenere plants
Autor(es): Cadavid-Labrada A, Medina C, Martinello L & Arce-Johnson P
Año: 2008. 1:73-74
Vitis
15 - Identification of genes expressed during early somatic embryogenesis in Pinus radiata
Autor(es): Aquea F and Arce-Johnson PI
Año: 2008. 46, 559-568
Plant Physiology and Biochemistry
16 - A novel Otubain-like cysteine protease gene is preferentially expressed during somatic embryogenesis in Pinus radiata.
Autor(es): Aquea F, Gutierrez F, Medina C & Arce-Johnson P
Año: 2008. 35(4): 567-573
Molecular Biology Reports
17 - Role of joule heating on the hydrodynamic boundary layer with rectangular electrodes - Numerical approach
Autor(es): Oyanader M and Arce P
Año: 2008. -
Latin American Applied Research
18 - The N-homologue LRR domain adopts a folding which explains the TMV-Cg-induced HR-like response in sensitive tobacco plants
Autor(es): Stange C, Dominguez C, Matus T, Perez T & Arce-Johnson P
Año: 2008. 26(5):850-60
Journal of Molecular Graphics and Modelling
19 - Structure-function relationship between the tobamovirus TMV-Cg coat protein and the HR-like response
Autor(es): Ehrenfeld N, Gonzalez A, Canon P, Medina C, Perez-Acle T & Arce-Johnson P
Año: 2008. 89: 800-817
Journal of Genetic Virology
20 - Phytoplasma and virus detection in commercial plantings of Vitis vinifera cv. Merlot exhibiting premature berry dehydration
Autor(es): Matus J, Vega A, Loyola R, Serrano C, Cabrera S & Arce-Johnson P
Año: 2008. 11 (5): 1-10
Electronic Journal of Biotechnology

21 - Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes
Autor(es): Matus JT, Aquea F & Arce-Johnson P
Año: 2008. 22, 8: 83
BMC Plant Biol
22 - Synthetic seed production from somatic embryos of Pinus radiata
Autor(es): Aquea F, Poupin MJ, Matus JT, Gebauer M, Medina C & Arce-Johnson P
Año: 2008. 10: 1847-52
Biotechnology Letters
23 - Senescence-associated genes induced during compatible viral interactions with grapevine and Arabidopsis
Autor(es): Espinoza C, Medina C, Somerville S & Arce-Johnson P
Año: 2007. 58(12) 3197-212
Journal of Experimental Botany
24 - Analysis of gene expression associated with compatible viral diseases in grapevine cultivars
Autor(es): Espinoza C, Vega A, Medina C, Schlauch K, Cramer G & Arce-Johnson P

Año: 2007. 7 (2), 95-110
Functional and Integrative Genomics
25 - Isolation of the three grape sub-lineages of B-class MADS-box TM6, PISTILLATA and APETALA 3 genes which are differentially expressed during flower and fruit development
Autor(es): Poupin J, Federici F, Medina C, Matus J, Timmermann T & Arce-Johnson P
Año: 2007. 404 (1-2): 10-24.
Gene
26 - The movement protein of crucifer-infecting TMV-Cg expressed in tobacco plants complements in trans a TMV-U1 trafficking deficient-mutant
Autor(es): Diaz-Griffero F, Espinoza C, Medina C & Arce-Johnson P
Año: 2006. 39: 269-279
Biological Research
27 - Tobamovirus coat protein CPCg induces an HR-like response in sensitive tobacco plants
Autor(es): Erhenfeld N, Cañon P, Stange C, Medina C & Arce-Johnson P
Año: 2005. 19(3): 418-427
Molecules and Cells
28 - Trangenics trees for a new era. In Vitro Cell
Autor(es): Poupin MJ and Arce-Johnson P
Año: 2005. 41: 91-101.
Developmental Biology
29 - Identification and characterization of a novel TMV resistance N gene homologue in Nicotiana tabacum plants
Autor(es): Stange C, Matus T, Elorza A & Arce-Johnson P
Año: 2004. 31: 149-158.
Functional Plant Biology
30 - Replicase mediated resistance against Potato Leafroll Virus in potato Desiree plants
Autor(es): Ehrenfeld N, Serrano C & Arce-Johnson P
Año: 2004. 37 (1): 71-82.
Biological Research

31 - Analysis of local and systemic spread of the Crucifer infecting TMV-Cg virus in tobacco and several Arabidopsis ecotypes
Autor(es): Arce-Johnson P, Padget H, Medina C, Huanca W & Espinoza C
Año: 2003. 30: 401-408.
Functional Plant Biology
32 - Stable transformation of embriogenic tissue of Pinus radiata by Agrobacterium tumefaciens
Autor(es): Cerda F, Ferrada C, Aquea F & Arce-Johnson P
Año: 2002. 70: 251-257.
Plant Cell Tissue and Organ Culture
33 - El debate de los transgenicos: una perspectiva desde la ciencia
Autor(es): Arce Johnson P and Vicuña R
Año: 2001. 87: 63-80.
Diplomacia
34 - Evaluation of three detection methods for TMV-Cg and TMV-U1 in Arabidopsis thaliana plants growing in vitro
Autor(es): Pereda S, Erherfeld N, Medina C & Arce-Johnson P
Año: 2000. 90 (2): 135-142.
Journal of Virology Methods
35 - Expression of the chicken lysozyme gene in potato enhances resistance to infection by Erwinia carotovora subsp. Atroseptica
Autor(es): Serrano C, Arce P, Torres H, Gebauer M, Gutierrez M, Moreno M, Jordana X, Venegas A, Kalazich J & Holuigue L
Año: 2000. 77: 191-199.
American Journal of Potato Research
36 - Optimization of in vitro culture conditions for Pinus radiata embryos and characterizations of the regenerated shoots
Autor(es): Stange C, Prehn D, Gebauer M & Arce-Johnson P
Año: 1999. 32: 19-28.
Biological Research
37 - Enhanced resistance to bacterial infection by Erwinia carotovora subsp. Atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes
Autor(es): Arce P, Moreno M, Gutierrez M, Gebauer M, Dell'Orto P, Torres H, Acuña I, Oliger P, Venegas A, Jordana X, Kalazich J & Holuigue L
Año: 1999. 75: 1-9.
American Journal of Potato Research
38 - Isolation of Pinus radiata genomic DNA suitable for RAPD analysis
Autor(es): Stange C, Prehn D & Arce-Johnson P
Año: 1998. (1) 1-8.
Plant Molecular Biology Reporter
39 - Proteins from the Gram-positive bacteria Clavibacter michiganensis subsp. michiganenesis, induce a hypersensitive-like response in plants
Autor(es): Alarcon C, Castro J, Muñoz F, Arce-Johnson P & Delgado J
Año: 1998. 88 (4) 306-310.
Phytopathology
40 - Requirement of the movement protein for long distance spread of tobacco mosaic virus in grafted plants
Autor(es): Arce-Johnson P, Reimann-Philipp U, Padgett H, Rivera R & Beachy RN
Año: 1997. 10 (6): 691-699.
Molecular Plant Microbe Interactions

Patricio Arce

Fono oficina: (56-2) 354-2897Laboratorio: (56-2) 354-2893Ubicación oficina: Edificio 210, Piso 7

Profesor Asociado

Estudios

Premios y Distinciones

Cursos

Líneas de Investigación

CARACTERIZACION GENETICA FISIOLOGICA Y MOLECULAR DE MICROALGAS PARA PRODUCCION DE BIOCOMBUSTIBLE

EFECTO DE LAS INFECCIONES VIRALES EN LA REGULACION DE BIOSINTESIS DE FLAVONOIDES MEDIADA POR AZUCARES, POR FACTORES DE TRANSCRIPCION MYB

GENÓMICA FUNCIONAL PARA EL ESTUDIO DE LA INTERACCIÓN VIRUS Y VIDES

FORTALECIMIENTO DE LA BIOLOGIA VEGETAL

GENOMICA FUNCIONAL VEGETAL

MEJORAMIENTO GENETICO DE LA VID

CONSORCIO PARA LA INNOVACIÓN DE LA INDUSTRIA DEL VINO: PLANTAS LIBRES DE VIRUS

1 - Compatible GLRaV-3 viral infection inhibits berry ripening by decreasing sugar accumulation and anthocianin biosynthesis in Vitis vinifera

2 - Genome wide analysis of the set domain group family in grapevine

3 - Biochemical and molecular changes in response to aluminum-stress in highbush blueberry (Vaccinum corymbosum L.)

4 - Long term protection against tobacco mosaic virus induced by the marine alga oligosulphated poly-ga in tobacco plants

5 - Identification of aluminium-regulated genes by c-DNA AFLP analysis in roots of two contrasting genotypes of highbush blueberry (Vaccinum corymbosum L.)

6 - Molecular and physiological strategies to increase aluminum resistance in plants

7 - Relationship between pollination and fertilization with the expression of genes regulating floral transition, hormone synthesis and early berry development in grapevine (Vitis vinifera L.)

8 - Analysis of histone acetyltransferase and deacetylase families of Vitis vinifera

9 - Isolation of WDR and bHLH genes related to flavonoid synthesis in grapevine (Vitis vinifera L.)

10 - TRAUCO, a Trithorax-group gene homologue, is required for early embryogenesis in Arabidopsis thaliana

11 - Mapping aluminum tolerance loci in cereals: A tool available for crop breeding

12 - Molecular characterization of a Trithorax-group homologue gene from Pinus radiata.

13 - Post-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera

14 - Somatic embryogenesis and efficient regeneration of Vitis vinifera L., cv. Carmenere plants

15 - Identification of genes expressed during early somatic embryogenesis in Pinus radiata

16 - A novel Otubain-like cysteine protease gene is preferentially expressed during somatic embryogenesis in Pinus radiata.

17 - Role of joule heating on the hydrodynamic boundary layer with rectangular electrodes - Numerical approach

18 - The N-homologue LRR domain adopts a folding which explains the TMV-Cg-induced HR-like response in sensitive tobacco plants

19 - Structure-function relationship between the tobamovirus TMV-Cg coat protein and the HR-like response

20 - Phytoplasma and virus detection in commercial plantings of Vitis vinifera cv. Merlot exhibiting premature berry dehydration

21 - Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes

22 - Synthetic seed production from somatic embryos of Pinus radiata

23 - Senescence-associated genes induced during compatible viral interactions with grapevine and Arabidopsis

24 - Analysis of gene expression associated with compatible viral diseases in grapevine cultivars

25 - Isolation of the three grape sub-lineages of B-class MADS-box TM6, PISTILLATA and APETALA 3 genes which are differentially expressed during flower and fruit development

26 - The movement protein of crucifer-infecting TMV-Cg expressed in tobacco plants complements in trans a TMV-U1 trafficking deficient-mutant

27 - Tobamovirus coat protein CPCg induces an HR-like response in sensitive tobacco plants

28 - Trangenics trees for a new era. In Vitro Cell

29 - Identification and characterization of a novel TMV resistance N gene homologue in Nicotiana tabacum plants

30 - Replicase mediated resistance against Potato Leafroll Virus in potato Desiree plants

31 - Analysis of local and systemic spread of the Crucifer infecting TMV-Cg virus in tobacco and several Arabidopsis ecotypes

32 - Stable transformation of embriogenic tissue of Pinus radiata by Agrobacterium tumefaciens

33 - El debate de los transgenicos: una perspectiva desde la ciencia

34 - Evaluation of three detection methods for TMV-Cg and TMV-U1 in Arabidopsis thaliana plants growing in vitro

35 - Expression of the chicken lysozyme gene in potato enhances resistance to infection by Erwinia carotovora subsp. Atroseptica

36 - Optimization of in vitro culture conditions for Pinus radiata embryos and characterizations of the regenerated shoots

37 - Enhanced resistance to bacterial infection by Erwinia carotovora subsp. Atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes

38 - Isolation of Pinus radiata genomic DNA suitable for RAPD analysis

39 - Proteins from the Gram-positive bacteria Clavibacter michiganensis subsp. michiganenesis, induce a hypersensitive-like response in plants

40 - Requirement of the movement protein for long distance spread of tobacco mosaic virus in grafted plants

41 - The amount of movement protein produced in transgenic plants influences the establishment, local movement, and systemic spread of infection by movement protein-deficient TMV

Fono oficina: (56-2) 354-2897
Laboratorio: (56-2) 354-2893
Ubicación oficina: Edificio 210, Piso 7