Miguel Bronfman

Fono oficina: (56-2) 354-2830
Laboratorio: (56-2) 354-2839
Ubicación oficina, Edificio Marcoleta 3° piso

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Profesor Titular

Unidad de Bioquímica Celular y Genética

Departamento de Biología Celular y Molecular,

Facultad de Ciencias Biológicas,

Pontificia Universidad Católica de Chile, Alameda 340, Santiago

Estudios

Químico, P. Universidad Católica de Chile, 1970.

Ph.D Institutefor Cell and Molecular Pathology, Faculty of Medicine, Universidad de Lovaina, Bélgica.(1976).

Post-Doctorado University of Wisconsin-Madison, Dep. Biochemistry (1977)

Curso que dicta: Biología Celular

Premios y Distinciones

Premio Poerre Bruylants, Universidad de Lovaina, Bélgica.
Premio Sociedad Médica de Santiago (1985)
Cátedra Presidencial en Ciencias. (1998)

Cursos

Pregrado

BIO266D

Laboratorio Bioquímica I: Biología Celular

Líneas de Investigación:

En nuestro laboratorio estamos interesados en entender el rol de los PPARs (Peroxisome proliferator activated receptors) en la regulación de la diferenciación celular y de la homeostasis lipídica. Estos receptores, activados por ligando, pertenecen a la súper familia de los receptores nucleares y son importantes en varias patologías como obesidad, diabetes y cáncer. Son el blanco de los proliferadores peroxisomales, un tipo de carcinógenos no-genotóxicos, tales como diversas drogas hipolipidémicas, plastificantes, herbicidas y otros contaminantes ambientales, y de antidiabéticos como las tiazolendionas.

Los receptores de hormonas de la super familia de los receptores nucleares son factores de transcripción que se unen a secuencias blanco de la región reguladora de genes sensibles a hormonas para aumentar o reprimir su transcripción. Tienen regiones muy conservadas evolutivamente consistentes en una serie de dominios estructurales discretos, que incluyen el dominio de unión a ligando, un dominio de dimerización y uno o más de transactivación. Los miembros de esta familia son importantes blancos en el desarrollo de fármacos, y tienen especial relevancia en una gran variedad de patologías menores y de alto impacto, que van desde acne (retinoides) a tumor de mama (receptor de estrógenos)

Los PPARs son los miembros más recientemente descubiertos de la familia de receptores nucleares. Son activados por ácidos grasos y sus metabolitos (eicosanoides), y controlan una gran variedad de genes blanco envueltos en etapas clave del metabolismo lipídico y de la diferenciación celular. Se han identificado varios subtipos de PPAR en diferentes especies de vertebrados. En base al análisis del dominio de unión a DNA, altamente conservados, los PPARs han sido clasificados en la sub familia de receptores nucleares que incluyen al receptor de ácido retinoico (RAR), de la hormona tiroídea, y de los receptores huérfanos (sin ligando conocido). La familia de los PPARs esta compuesta de tres subtipos, PPARa, PPARb y PPARg. Todos forman heterodímeros con el receptor de retinoides X (RXR). El PPARa modula la acción pleiotrópica de los proliferadores peroxisomales, regulando genes de la homeostasis lipídica, tales como los del metabolismo de ácidos grasos en mitocondrias, peroxisomas, y citocromo P450, la síntesis de apolipoproteínas, transporte intracellular de lípidos y supresión de la apoptosis. El PPARg afecta vías importantes en una serie de enfermedades, mediante acciones que median la diferenciación de varios tipos celulares no relacionados. Estas incluyen adipositos, hepatocitos, fibroblastos, miocitos, células de mama y epiteliales de colon, y macrófagos/monocitos. Poco se conoce aún de la función de PPARb.

Actualmente, nuestro interés se centra principalmente en la identificación de ligandos fisiológicos de PPAR, en la interacción de estos factores de transcripción con otras vías de señalización y en su función en células del sistema nervioso.

1 - Megalin/LRP2 expression is induced by peroxisome proliferator-activated receptor -alpha and -gamma: implications for PPARs' roles in renal function
Autor(es): Cabezas F, Lagos J, Cespedes C, Vio CP, Bronfman M & Marzolo MP
Año: 2011. 6(2): p. e16794.
PLOS One
2 - Rosiglitazone treatment prevents mitochondrial dysfunction in mutant huntingtin-expressing cells: possible role of peroxisome proliferator-activated receptor-gamma (PPARgamma) in the pathogenesis of Huntington disease.
Autor(es): Quintanilla RA, Yin YN, Fuenzalida K, Bronfman M & Johnson GV
Año: 2008. 283(37): p. 25628-37.
Journal of Biological Chemistry
3 - A PPAPRs cross-talk concertedly commits c6 giloma cells to oligodendrocytes and induces enzymes involved in myelin synthesis
Autor(es): Leisewitz AV, Urrutia CR, Martinez GR, Loyola G & Bronfman M
Año: 2008. In press
Journal of Cell Physiology
4 - PPAR gamma up-regulates the Bcl-2 anti-apoptotic protein in neurons and induces mitochondrial stabilization and protection against oxidative stress and apoptosis
Autor(es): Fuenzalida K, Quintanilla R, Ramos P, Piderit D, Fuentealba RA, Martinez GR, Inestrosa NC & Bronfman M
Año: 2007. 282(51):37006-15.
Journal of Biological Chemistry
5 - P450 CYP2C epoxygenase and CYP4A omega-hydroxylase mediate ciprofibrate-induced PPARalpha-dependent peroxisomal proliferation
Autor(es): Gatica A, Aguilera MC, Contador D, Loyola G, Pinto CO, Amigo L, Tichauer JE, Zanlungo S & Bronfman M
Año: 2007. 48(4):924-34
Journal of Lipid Research
6 - Inhibition of NF-kB enhances the capacity of immature dendritic cells to induce antigen-specific tolerance in experimental autoimmune encephalomyelitis
Autor(es): Iruretagoyena MI, Sepulveda SE, Lezana JP, Hermoso M, Bronfman M, Gutierrez MA, Jacobelli SH & Kalergis AM
Año: 2006. 318: 59-67
Journal of Pharmacology and Experimental Therapeutics
7 - Peroxisome proliferator-activated receptor gamma is expressed in hippocampal neurons and its activation prevents beta-amyloid neurodegeneration: role of Wnt signaling
Autor(es): Inestrosa NC, Godoy JA, Quintanilla RA, Koenig CS & Bronfman M
Año: 2005
Experimental Cell Research
8 - Wnt-3a overcomes beta-amyloid toxicity in rat hippocampal neurons
Autor(es): Alvarez AR, Godoy JA, Mullendorff K, Olivares GH, Bronfman M & Inestrosa NC
Año: 2004. 297 (1) :186-96.
Experimental Cell Research
9 - Signal Transduction during amyloid-beta-peptide neurotoxicity: role in Alzheimer disease
Autor(es): Fuentealba RA, Farias G, Scheu J, Bronfman M, Marzolo MP & Inestrosa NC
Año: 2004. 47 (1-3) :275-89.
Brain Research Reviews
10 - Activation of Wnt signaling rescue neurodegeneration and behavioral impairments induced by b-amyloid fibrils
Autor(es): De Ferrari GV, Chacon MA, Garrido JL, Godoy JA, Alvarez A, Barria MI, Olivares GH, Reyes AE, Bronfman M & Inestrosa NC
Año: 2003. 280 (10) : 9604-9.
Molecular Psychiatry

11 - PPAR gamma activators induce growth arrest and process extension in B12 oligodendrocyte-like cells and terminal differentiation of cultured oligodendrocytes
Autor(es): Roth AD, Leisewitz AV, Jung JE, Cassina P, Barbeito L, Inestrosa NC & Bronfman M
Año: 2003
Journal of Neuroscience
12 - Ethanol specifically decreases peroxisome proliferator activated receptor beta in B12 oligodendrocyte-like cells
Autor(es): Leisewitz AV, Jung JE, Perez-Alzola P, Fuenzalida KM, Roth A, Inestrosa NC & Bronfman M
Año: 2003
Journal of Neurochemistry
13 - The hypolipidemic drug metabolites nafenopin-CoA and ciprofibroyl-CoA are competitive P2Y1 receptor antagonists
Autor(es): Coddou C, Loyola G, Boyer JL, Bronfman M & Huidobro-Toro JP
Año: 2003
FEBS Letters
14 - Wnt signaling involvement in b-amyloid-dependent neurodegeneration
Autor(es): Inestrosa NC, De Ferrari GV, Garrido J, Alvarez A, Olivares G, Barria M, Bronfman M & Chacon M
Año: 2002
Neurochemistry International
15 - Protein kinase C inhibits amyloid beta peptide neurotoxicity by acting on members of the Wnt pathway
Autor(es): Garrido JL, Godoy JA, Alvarez A, Bronfman M & Inestrosa NC
Año: 2002
FASEB Journal
16 - Multitest II: a program for the generation, correction and analysis of multiple chioce test
Autor(es): Bronfman M, Loyola G & Koenig CS
Año: 1998
Analytical Biochemistry
17 - Hypolipidaemic drugs are activated to acyl-Coenzyme A esters in isolated hepatocytes. Detection of drug activation by human liver homogenates and by human platelets
Autor(es): Bronfman M, Ponce C, Rojas S, Roth A, Loyola G, Vollrath V & Chianale J
Año: 1998
European Journal of Cell Biology
18 - Induction of peroxisomal fatty acyl-coenzyme A oxidase and total carnitine acetyl-Coenzyme A transferase in primary cultures of rat hepatocytes by garlic extracts
Autor(es): Orellana A, Kawada ME, Morales MN, Vargas L & Bronfman M

Año: 1997
Journal of Hepatology
19 - Subcellular distribution and characteristics of ciprofibroyl-CoA synthetase in rat liver: its possible identity with long chain acyl-CoA synthetase
Autor(es): Urrea R and Bronfman M
Año: 1996
FEBS Letters
20 - Saturable binding sites for the coenzyme A esters of nafenopin, a peroxisome proliferator, in rat liver cytosol
Autor(es): Bronfman M, Nuñez L, Orellana A, Burzio V & Hidalgo P
Año: 1996
Annals of the New York Academy of Sciences

21 - Microbial removal of chlorinated phenols during aerobic treatment of effluents from radiata pine kraft pulps bleached with chlorine-based chemicals, with or without hemicellulases
Autor(es): Cespedes R, Maturana A, Bumann U, Bronfman M & Gonzalez B
Año: 1996
Archives of Microbiology & Biotecnology
22 - Peroxisome proliferators and signal transduction
Autor(es): Morgan C and Bronfman M
Año: 1995 804: 403-411.
Xenobiótica
23 - Ciprofibrate, a carcinogenic peroxisome proliferator, increases phosphorylation of epidermal-growth-factor receptor in isolated rat hepatocytes
Autor(es): Orellana A, Holuigue L, Hidalgo PC, Faundez V, Gonzalez A & Bronfman M
Año: 1993. 215: 903-906.
Journal of Biological Chemistry
24 - Species differences in the intracellular distribution of ciprofibroyl-CoA hydrolase. Implications for peroxisome proliferation
Autor(es): Amigo L, McElroy MC, Morales MN & Bronfman M
Año: 1992
Biochemical Journal
25 - Overexpression of mdr2 gene by peroxisome proliferators in the mouse liver
Autor(es): Orellana A, Kawada ME, Morales MN, Vargas L & Bronfman M
Año: 1992
Toxicology Letters
26 - Enhanced differentiation of HL-60 leukemia cells to macrophages induced by ciprofibrate
Autor(es): Bronfman M, Morales MN, Amigo L, Orellana A, Nuñez L, Cardenas L & Hidalgo PC
Año: 1992
Biochemical Journal
27 - Isolation of intact organelles by differential centrifugation of digitonin-treated hepatocytes using a table eppendorf centrifuge
Autor(es): Lira P, Bronfman M & Eyzaguirre J
Año: 1990
IEEE Transactions on Education
28 - Palmitoyl-CoA and the acyl-CoA thioester of the carcinogenic peroxisome-proliferator ciprofibrate potentiate diacylglycerol- activated protein kinase C by decreasing the phosphatidylserine requirement of the enzyme
Autor(es): Orellana A, Hidalgo PC, Morales MN, Mezzano D & Bronfman M
Año: 1990
European Journal of Biochemistry
29 - Potentiation of diacylglycerol-activated protein kinase C by acyl-Coenzyme A thioesters of hypolipidaemic drugs
Autor(es): Bronfman M, Orellana A, Morales MN, Bieri F, Waetcher F, Staubli W & Bentley P
Año: 1989
Biochemical and Biophysical Research Communications
30 - Diacylglycerol activation of protein kinase C is modulated by long-chain acyl-CoA
Autor(es): Bronfman M, Morales MN & Orellana A
Año: 1988
Biophysical Research Comunication

31 - Quantitative Subcelullar study of apical pole membranes from Chicken Oxyntic Cells in Resting and HCl secretory state
Autor(es): Koenig C, Dabike M & Bronfman M
Año: 1987
Journal of Cell Biology
32 - Activation of Hypolipidemic drugs to acyl-coenzyme A thioesters
Autor(es): Bronfman M, Amigo L & Morales MN
Año: 1986
Biochemical Journal
33 - Carnitine acyltransferase and acyl-coenzyme A hydrolase activities in human liver. Quantitative analysis of their subcellular localization
Autor(es): Bronfman M and Leighton F
Año: 1984
Biochemical Journal
34 - Selective inhibition of peroxisomal fatty acid oxidation by phenothiazines, in membrane biogenesis and recycling, biological and medical aspects
Autor(es): Nicovani S, Orellana A, Necochea C, Bronfman M & Leighton F
Año: 1984
Biological and Medical Aspects
35 - Regulation of Biliary Cholesterol Secretion in the Rat. Role of hepatic cholesterol esterification.
Autor(es): Nervi O, Bronfman M, Allalon W, Depiereux E & Del R
Año: 1984
Journal of Clinical Investigation
36 - Palmitoyl-CoA synthetase and the peroxisomal system of B-oxidation in human livers. Quantitative analysis of the subcellular localization
Autor(es): Bronfman M, Inestrosa NC, Nervi FO & Leighton F
Año: 1984
Biochemical Journal
37 - Efecto de la Diosgenina sobre biosintesis de Colesterol, colesterol ester, Fosfolipidos, trigliceridos y sales biliares en hepatocitos aislados de rata
Autor(es): Alallon W, Nervi F, Bronfman M & Leighton F
Año: 1983
Revista Uruguaya de Patologia Clinica
38 - Subcellular fractionations on theorganization of the fatty acid oxidations by liver peroxisomes
Autor(es): Leighton F, Brandan E, Lazo O & Bronfman M
Año: 1982
Annals of the New York Academy of Sciences
39 - Isolation of peroxisomes by isopycnic density centrifugation in metrizamide gradients and analysis of the distribution of constituents with multiple localization by constrained linear regression
Autor(es): Bronfman M, Leighton F & Feymans E
Año: 1982
Annals of the New York Academy of Sciences
40 - Subcellular distribution of Cholesterol ester hydrolase in human liver
Autor(es): Del Pozo R, Bronfman M, Bull P & Nervi F
Año: 1981
IRCS Medical Sciences

Miguel Bronfman

Fono oficina: (56-2) 354-2830 Laboratorio: (56-2) 354-2839 Ubicación oficina, Edificio Marcoleta 3° piso

Profesor Titular

Unidad de Bioquímica Celular y Genética

Estudios

Premios y Distinciones

Cursos

Líneas de Investigación:

XVI CONCURSO DE I D FONDEF 2008

Interacción entre las Vías de Señalización de TGF-β y PPARγ en la Diferenciación de Células Madres provenientes de Tejido adiposo humano a Línea Condroídea

1 - Megalin/LRP2 expression is induced by peroxisome proliferator-activated receptor -alpha and -gamma: implications for PPARs' roles in renal function

2 - Rosiglitazone treatment prevents mitochondrial dysfunction in mutant huntingtin-expressing cells: possible role of peroxisome proliferator-activated receptor-gamma (PPARgamma) in the pathogenesis of Huntington disease.

3 - A PPAPRs cross-talk concertedly commits c6 giloma cells to oligodendrocytes and induces enzymes involved in myelin synthesis

4 - PPAR gamma up-regulates the Bcl-2 anti-apoptotic protein in neurons and induces mitochondrial stabilization and protection against oxidative stress and apoptosis

5 - P450 CYP2C epoxygenase and CYP4A omega-hydroxylase mediate ciprofibrate-induced PPARalpha-dependent peroxisomal proliferation

6 - Inhibition of NF-kB enhances the capacity of immature dendritic cells to induce antigen-specific tolerance in experimental autoimmune encephalomyelitis

7 - Peroxisome proliferator-activated receptor gamma is expressed in hippocampal neurons and its activation prevents beta-amyloid neurodegeneration: role of Wnt signaling

8 - Wnt-3a overcomes beta-amyloid toxicity in rat hippocampal neurons

9 - Signal Transduction during amyloid-beta-peptide neurotoxicity: role in Alzheimer disease

10 - Activation of Wnt signaling rescue neurodegeneration and behavioral impairments induced by b-amyloid fibrils

11 - PPAR gamma activators induce growth arrest and process extension in B12 oligodendrocyte-like cells and terminal differentiation of cultured oligodendrocytes

12 - Ethanol specifically decreases peroxisome proliferator activated receptor beta in B12 oligodendrocyte-like cells

13 - The hypolipidemic drug metabolites nafenopin-CoA and ciprofibroyl-CoA are competitive P2Y1 receptor antagonists

14 - Wnt signaling involvement in b-amyloid-dependent neurodegeneration

15 - Protein kinase C inhibits amyloid beta peptide neurotoxicity by acting on members of the Wnt pathway

16 - Multitest II: a program for the generation, correction and analysis of multiple chioce test

17 - Hypolipidaemic drugs are activated to acyl-Coenzyme A esters in isolated hepatocytes. Detection of drug activation by human liver homogenates and by human platelets

18 - Induction of peroxisomal fatty acyl-coenzyme A oxidase and total carnitine acetyl-Coenzyme A transferase in primary cultures of rat hepatocytes by garlic extracts

19 - Subcellular distribution and characteristics of ciprofibroyl-CoA synthetase in rat liver: its possible identity with long chain acyl-CoA synthetase

20 - Saturable binding sites for the coenzyme A esters of nafenopin, a peroxisome proliferator, in rat liver cytosol

21 - Microbial removal of chlorinated phenols during aerobic treatment of effluents from radiata pine kraft pulps bleached with chlorine-based chemicals, with or without hemicellulases

22 - Peroxisome proliferators and signal transduction

23 - Ciprofibrate, a carcinogenic peroxisome proliferator, increases phosphorylation of epidermal-growth-factor receptor in isolated rat hepatocytes

24 - Species differences in the intracellular distribution of ciprofibroyl-CoA hydrolase. Implications for peroxisome proliferation

25 - Overexpression of mdr2 gene by peroxisome proliferators in the mouse liver

26 - Enhanced differentiation of HL-60 leukemia cells to macrophages induced by ciprofibrate

27 - Isolation of intact organelles by differential centrifugation of digitonin-treated hepatocytes using a table eppendorf centrifuge

28 - Palmitoyl-CoA and the acyl-CoA thioester of the carcinogenic peroxisome-proliferator ciprofibrate potentiate diacylglycerol- activated protein kinase C by decreasing the phosphatidylserine requirement of the enzyme

29 - Potentiation of diacylglycerol-activated protein kinase C by acyl-Coenzyme A thioesters of hypolipidaemic drugs

30 - Diacylglycerol activation of protein kinase C is modulated by long-chain acyl-CoA

31 - Quantitative Subcelullar study of apical pole membranes from Chicken Oxyntic Cells in Resting and HCl secretory state

32 - Activation of Hypolipidemic drugs to acyl-coenzyme A thioesters

33 - Carnitine acyltransferase and acyl-coenzyme A hydrolase activities in human liver. Quantitative analysis of their subcellular localization

34 - Selective inhibition of peroxisomal fatty acid oxidation by phenothiazines, in membrane biogenesis and recycling, biological and medical aspects

35 - Regulation of Biliary Cholesterol Secretion in the Rat. Role of hepatic cholesterol esterification.

36 - Palmitoyl-CoA synthetase and the peroxisomal system of B-oxidation in human livers. Quantitative analysis of the subcellular localization

37 - Efecto de la Diosgenina sobre biosintesis de Colesterol, colesterol ester, Fosfolipidos, trigliceridos y sales biliares en hepatocitos aislados de rata

38 - Subcellular fractionations on theorganization of the fatty acid oxidations by liver peroxisomes

39 - Isolation of peroxisomes by isopycnic density centrifugation in metrizamide gradients and analysis of the distribution of constituents with multiple localization by constrained linear regression

40 - Subcellular distribution of Cholesterol ester hydrolase in human liver

41 - Fatty acid oxidation by human liver peroxisomes

42 - Detection of peroxisomal fatty-acyl CoA oxidase activity

43 - Properties of fatty acyl-CoA oxidase from rat liver, a peroxisomal flavoprotein.

44 - The mechanism of ATP depletion in the liver after a load of fructose

45 - Stress,Insulin antagonist and transient diabetes mellitus in the rat

46 - Alteration of Subcellular organelles induced by compression

47 - Rol de la secrecion gastrica en la absorcion de fierro,Parte I

48 - Rol de la secrecion gastrica en la absorcion de fierro,Parte II

Fono oficina: (56-2) 354-2830
Laboratorio: (56-2) 354-2839
Ubicación oficina, Edificio Marcoleta 3° piso